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Accession number;99A0228223
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| Title;Surface Forces between Two-Dimensionally Organized Sigma A-Spo0A Protein Layers. |
| Author;
KURIHARA KAZUE
(Tohoku Univ.)
HAMAI YASUHIRO
(Tohoku Univ.)
SASAKI D Y
(Sandia National Lab.)
FUJITA MASAYA
(National Inst. of Genetics)
SADAIE YOSHITO
(National Inst. of Genetics)
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Journal Title;Abstracts. Symposium on Biofunctional Chemistry
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Journal Code:L0836A
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ISSN:
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VOL.13th;NO.;PAGE.103-105(1998)
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| Figure&Table&Reference; |
| Pub. Country;Japan |
| Language;Japanese |
| Abstract;Two-dimensionally organized protein layers were prepared utilizing Langmuir-Blodgett(LB) films which could bind His-tag (poly(histidine) tagged) proteins through specific interaction between metal-chelating lipids and poly(histidine) stretches via Cu(II) ions. Colloidal probe atomic force microscopy was employed to measure forces between these protein monolayers. Protein used in this study were Sigma A subunit of RNA polymerase and a transcrition factor Spo0A. Interactions between Sigma A-Sigma A, Spo0A-Spo0A, and Sigma A-Spo0A were investigated. (author abst.) |
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