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Accession number;99A0228227
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| Title;Viscosity Effects of Reaction Medium on Rate of Reactions Catalyzed by Hydrolases. (4). |
| Author;
KAWAI YASUSHI
(Kyoto Univ.)
MATSUO TAKASHI
(Kyoto Univ.)
ONO ATSUYOSHI
(Kyoto Univ.)
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Journal Title;Abstracts. Symposium on Biofunctional Chemistry
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Journal Code:L0836A
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ISSN:
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VOL.13th;NO.;PAGE.115-117(1998)
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| Figure&Table&Reference; |
| Pub. Country;Japan |
| Language;Japanese |
| Abstract;Conformational change of enzyme at the rate-determining step plays an important role in an acceleration of enzyme-catalyzed reactions, which can be explained by "induced-fit" hypothesis. In order to elucidate the significance of the conformational change of enzyme, we have investigated the effects of viscosity of reaction medium upon reaction rates of hydrolysis of p-nitrophenyl esters by .ALPHA.-chymotrypsin. The effects of viscosity on kinetic parameters is substrate dependent, which shows that conformational change of the enzyme depends upon the structure of a substrate. The viscosity-kinetics profile in sucrose solution is identical with that in glycerol solution, which shows that the behavior of kinetic parameters observed stems not from the specific effects of a viscogen but from the medium viscosity. The effects of medium viscosity on hydrolysis of p-nitroanilides of N-protected amino acid is under investigation. (author abst.) |
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