Science Links Japan | Isolation of Shiga Toxin-producing Escherichia coli O157:H7 from Processed Salmon Roe Associated with the Outbreaks in Japan, 1998, and a Molecular Typing of the Isolates by Pulsed-field Gel Electrophoresis.

Isolation of Shiga Toxin-producing Escherichia coli O157:H7 from Processed Salmon Roe Associated with the Outbreaks in Japan, 1998, and a Molecular Typing of the Isolates by Pulsed-field Gel Electrophoresis.

Accession number;99A0269804

Title;Isolation of Shiga Toxin-producing Escherichia coli O157:H7 from Processed Salmon Roe Associated with the Outbreaks in Japan, 1998, and a Molecular Typing of the Isolates by Pulsed-field Gel Electrophoresis.

Author; ASAI YOSHIO (Kanagawa Prefect. Public Health Lab.) MURASE TOSHIYUKI (Kanagawa Prefect. Public Health Lab.) SUZUKI RIEKO (Kanagawa Prefect. Public Health Lab.) SATA SHIN (Kanagawa Prefect. Public Health Lab.) YAMAI SHIRO (Kanagawa Prefect. Public Health Lab.) TERAJIMA JUN (Kansenshoken) IZUMIYA HIDEMASA (Kansenshoken) TAMURA KAZUMICHI (Kansenshoken) WATANABE HARUO (Kansenshoken)

Journal Title;Journal of the Japanese Association for Infectious Diseases

Journal Code:Z0760A

ISSN:0387-5911

VOL.73;NO.1;PAGE.20-24(1999)

Figure&Table&Reference;FIG.1, TBL.1, REF.10

Pub. Country;Japan

Language;Japanese

Abstract;Shiga toxin-producing Escherichia coil(STEC) O157 were isolated from processed salmon roe which had been a suspected food item in sporadic infections which occurred in Japan in 1998. A total of 45 samples of the processed salmon roe were pre-enriched in trypticase soy broth(TSB) at 36.DEG.C. for 6h and novobiocin-supplemented modified EC both (mEC-NB) at 42.DEG.C. for 18h. After the pre-enrichments, the cultures were examined for possible occurrence of STEC O157, using an immunomagnetic separation(IMS) method. From the examination, a total of 84 strains of STEC O157:H7 that were positive for both stx 1 and stx 2 genes were isolated. By applying the most-probable-number technique, it was estimated that the number of STEC O157 was in the range of 0.73-1.5 per 10g of the processed salmon roe. Subsequent analysis of the isolates by a pulsed-field gel electrophoresis(PFGE) revealed a pattern commonly seen in 82 isolates and another pattern in two isolates. Clinical isolates from 7 patients also showed an identical pattern to those of the 82 isolates and one isolate from a patient showed the other pattern identical to those of the two isolates. The isolates were found to belong to the phage type 14. (author abst.)

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