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Accession number;99A0412431
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| Title;Large-scale Production of a High-glutamine Peptide. |
| Author;
TANABE SOICHI
(Tokyo Gakugei Univ., Fac. of Educ.)
ARAI SOICHI
(Tokyonodai Oyoseibutsukagaku)
WATANABE MICHIKO
(Tokyo Gakugei Univ., Fac. of Educ.)
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Journal Title;Journal of Japanese Society of Nutrition and Food Science
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Journal Code:F0624A
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ISSN:0287-3516
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VOL.52;NO.2;PAGE.103-106(1999)
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| Figure&Table&Reference;FIG.2, TBL.2, REF.11 |
| Pub. Country;Japan |
| Language;Japanese |
| Abstract;A practical method was developed for the large-scale production of a high-glutamine peptide mixture. A gluten ball was freeze-dried, pulverized, and passed through a 48-mesh screen to obtain a starting material. A 10-kg portion of this material as a substrate was mixed with 50 L of water containing 100 g of actinase AS, and the mixture was incubated at 40.DEG.C. at pH 7.0 for 1 h, with the result that about 90% of the substrate was solubilized. The soluble sample was subjected to Sephadex G-15 column chromatography to obtain an oligopeptide fraction with a Glx content of 50.6%. This was then treated with a membrane filter to remove microorganisms, giving a final product for enteral nutrition use. (author abst.) |
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