A Practical Method for Determination of Vitamin C in Plasma by High-performance Liquid Chromatography with an Electrochemical Detector.
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Accession number;99A0412432
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| Title;A Practical Method for Determination of Vitamin C in Plasma by High-performance Liquid Chromatography with an Electrochemical Detector. |
| Author;
UMEGAKI KEIZO
(National Inst. Health Andnutrition)
YOSHIMURA MIKA
(National Inst. Health Andnutrition)
NISHIMUTA MAMORU
(National Inst. Health Andnutrition)
ESASHI TAKATOSHI
(National Inst. Health Andnutrition)
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Journal Title;Journal of Japanese Society of Nutrition and Food Science
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Journal Code:F0624A
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ISSN:0287-3516
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VOL.52;NO.2;PAGE.107-111(1999)
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| Figure&Table&Reference;FIG.4, REF.12 |
| Pub. Country;Japan |
| Language;Japanese |
| Abstract;For analysis of ascorbic acid (AsA) using high-performance liquid chromatography (HPLC) with an electrochemical detector (ECD), we compared two different methods for preparation of the AsA sample. One used methaphosphoric acid (MPA) as a pretreatment solution for the AsA sample, and the other used EDTA-methanol, as reported previously (J Nutr Sci Vitaminol 40:73 (1994)). AsA in plasma treated with EDTA-methanol was unstable even when stored at 4.DEG.C., while that treated with MPA was very stable. After examining several HPLC conditions for the MPA-treated plasma sample, the best was found to be: mobile phase, 0.2 M KH2PO4-H3PO4 (pH 3.0) containing 0.5 mM dodecyltrimethylammonium chloride and 0.2 mM EDTA; flow rate, 1 mL/min; column, C18 (4.6*100 mm); applied voltage of ECD, +350 mV versus Ag/AgCl. Under this condition, dehydroascorbic acid (DAsA), which was reduced with dithiothreitol to AsA before being applied to HPLC, could be analyzed rapidly. (author abst.) |
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