Interleukin-6 (IL-6) Does Not Change the Expression of Bcl-2 Protein in the Prevention of Cisplatin-Induced Apoptosis in Ovarian Cancer Cell Lines.
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Accession number;99A0225423
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| Title;Interleukin-6 (IL-6) Does Not Change the Expression of Bcl-2 Protein in the Prevention of Cisplatin-Induced Apoptosis in Ovarian Cancer Cell Lines. |
| Author;
ISHIOKA S
(Sapporo Medical Univ., Sapporo, Jpn)
VAN HAAFTEN-DAY C
(Royal Hospital For Women, Nsw, Aus)
SAGAE S
(Sapporo Medical Univ., Sapporo, Jpn)
KUDO R
(Sapporo Medical Univ., Sapporo, Jpn)
HACKER N F
(Royal Hospital For Women, Nsw, Aus)
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Journal Title;J Obstet Gynaecol Res
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Journal Code:Y0696A
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ISSN:1341-8076
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VOL.25;NO.1;PAGE.23-27(1999)
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| Figure&Table&Reference;FIG.1, TBL.2, REF.15 |
| Pub. Country;Japan |
| Language;English |
| Abstract;Objective: To study whether interleukin-6 (IL-6) changes the expression of the antiapoptic Bcl-2 protein in the prevention of cis-diaminedichloroplatinum (II) (CDDP)-induced apoptosis of human ovarian cancer cells. Methods: Comparative studies were performed on 3 ovarian cancer cell lines after 48 hours of exposure to 0.5-50 ng/ml IL-6, 2 .MU.g/ml anti-IL-6 monoclonal antibody (anti-IL-6 mAb), 10 .MU.M CDDP, 10 .MU.M CDDP + 0.5-50 ng/ml IL-6, and 10 .MU.M CDDP + 2 .MU.g/ml anti-IL-6 mAb. Apoptosis was measured morphologically and by a DNA fragmentation assay. Bcl-2 protein levels were measured by an ELISA. Results: An increase in apoptosis was observed for each cell line after 48 hours of exposure to 10 .MU.M CDDP. Although high doses of IL-6 decreased the percentage of apoptotic cells, this cytokine did not change the expression of the Bcl-2 protein. Conclusion: CDDP-induced apoptosis was negatively controlled by IL-6. However, the anti-apoptic Bcl-2 protein level was not changed by IL-6 in the process of apoptosis in the ovarian cancer cell lines. (author abst.) |
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