Comparative Study of Quantitative Microscopic Analysis and Interphase Cytogenetics in Bladder Cancer.
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Accession number;99A0233524
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| Title;Comparative Study of Quantitative Microscopic Analysis and Interphase Cytogenetics in Bladder Cancer. |
| Author;
NEMOTO RYOSUKE
(Tottori Prefect. Cent. Hosp.)
NAKAMURA ISAO
(Tottori Prefect. Cent. Hosp.)
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Journal Title;Nishinihon Journal of Urology
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Journal Code:Z0253B
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ISSN:0029-0726
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VOL.61;NO.1;PAGE.22-26(1999)
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| Figure&Table&Reference;FIG.4, TBL.1, REF.19 |
| Pub. Country;Japan |
| Language;Japanese |
| Abstract;The relationship between quantitative microscopic analysis using image cytometry and interphase cytogenetics was investigated in 17 patients with transitional cell carcinomas of the urinary bladder. By use of in situ hybridization with chromosome-specific DNA probes, the copy number of pericentrometric sequences on chromosomes 1, 7, 10, 11, 17, 18 X and Y was detected within interphase nuclei in formalin-fixed and paraffin-embedded sections, the routinely processed from 17 patients with bladder cancers. The percentage of hyperdiploid cells (three or more spots) was estimated using light microscopy. The percentage of hyperdiploid cells for chromosomes 7 and 17 was highly correlated with increasing DNA index and mean nuclear area (p<0.05). The percentage of hyperdiploid cells for chromosomes 10 and Y was not correlated with either DNA index or nuclear area. Since a high DNA index is indicative of both a more aggressive tumor behavior and a worse prognosis, these findings suggest that the percentage of hyperdiploid cells, especially for chromosomes 7 and 17, may be highly predictive of bladder tumor aggressiveness, in addition to nuclear shape factors (p<0.05). Moreover, our preliminary results suggest that measurement of nuclear shape factors is affected by numerical chromosome aberrations and total nuclear DNA content. (author abst.) |
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