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Accession number;99A0460689
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| Title;High Inducibility of Epstein-Barr Virus Replication in B Lymphocytes in Vogt-Koyanagi-Harada Disease. |
| Author;
MINODA HIROSHI
(Tokyo Medical College)
SAKAI JUN'ICHI
(Tokyo Medical College)
SUGIURA MAKOTO
(Hokkaido Univ., Cancer Inst.)
IMAI SHOSUKE
(Hokkaido Univ., Cancer Inst.)
OSATO TOYORO
(Health Sci. Univ. Hokkaido)
USUI MASAHIKO
(Tokyo Medical College)
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Journal Title;Journal of Japanese Ophthalmological Society
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Journal Code:Z0666A
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ISSN:0029-0203
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VOL.103;NO.4;PAGE.289-296(1999)
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| Figure&Table&Reference;FIG.8, TBL.2, REF.25 |
| Pub. Country;Japan |
| Language;Japanese |
| Abstract;Purpose: The role of Epstein-Barr virus(EBV) reactivation in the pathogenesis of Vogt-Koyanagi-Harada(VKH) disease was examined. Material and Methods: Using B lymphocytes obtained from 8 patients with VKH disease and 10 patients with other types of uveitis, immortarized lymphoblast lines were established and infected with EBV. The degree of EBV activation in each lymphoblast line, in the presence and absence of various stimuli, was assessed by measuring the expression of 3 different antigens involved in replication by immunofluorescent staining and western blot analysis. Quantification of EBV DNA in cell culture supernatants was done by polymerase chain reaction. Result: Cell lines established from VKH patients expressed more viral antigens that those established from patients with other types of uveitis. There were greater amounts of EBV DNA in the VKH cell lines. Conclusion: B lymphocytes from VKH patients may be more susceptible to EBV activation, and the reactivation of EBV may be involved in the pathogenesis of VKH. (author abst.) |
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