Investigation of a New p24 Antigen Detection System by the Chemiluminescence-enzyme-immuno-assay.

Accession number;99A0447117
Title;Investigation of a New p24 Antigen Detection System by the Chemiluminescence-enzyme-immuno-assay.
Author; SAKAI ATSUKO (International Medical Center of Japan) HIRABAYASHI YOSHIHIRO (International Medical Center of Japan) AIZAWA SAORI (International Medical Center of Japan) TANAKA MARI (International Medical Center of Japan) IDA SETSUKO (International Medical Center of Japan) OKA SHIN'ICHI (International Medical Center of Japan)
Journal Title;Journal of the Japanese Association for Infectious Diseases
Journal Code:Z0760A
ISSN:0387-5911
VOL.73;NO.3;PAGE.205-212(1999)
Figure&Table&Reference;FIG.4, TBL.5, REF.9
Pub. Country;Japan
Language;Japanese
Abstract;We investigated a new p24 antigen detection system by the chemiluminescence-enzymeimmuno-assay(CLEIA) and compared it with the conventional antigen-kits(A;Abbott, B;Coulter). The detection limit of CLEIA, A, and B were 4.3pg/ml, 11.3pg/ml, and 5.6pg/ml, respectively. When the patent's sera and panel sera were examined by the three method, compatible results with the detection limit were obtained. CLEIA can be completed within 35min, and is performed by an automatic system, which can treat many samples at one time. Therefore, this system is more suitable for screening of donor blood because of the shortening of the window period. (author abst.)
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