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Accession number;99A0447118
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| Title;Rapid and Simple Method for the Detection of Shigatoxins(STxs). |
| Author;
KOBAYASHI KAZUHIRO
(Osaka Prefect. Inst. of Public Health)
TAGUCHI MASUMI
(Osaka Prefect. Inst. of Public Health)
SETO KAZUKO
(Osaka Prefect. Inst. of Public Health)
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Journal Title;Journal of the Japanese Association for Infectious Diseases
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Journal Code:Z0760A
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ISSN:0387-5911
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VOL.73;NO.3;PAGE.213-217(1999)
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| Figure&Table&Reference;FIG.1, TBL.3, REF.3 |
| Pub. Country;Japan |
| Language;Japanese |
| Abstract;Enterohemorrhagic Escherichia coli(EHEC) is an emerged bacterial agent as the cause of bloody diarrhea and a leading cause of hemolytic uremic syndrome in children. In our country, serotype O157:H7 is the predominant pathogen in the EHEC and the most frequently seen in human infections. The clinical disease is not associated with the STx types produced by the infecting strains. Non-O157 serotypes of EHEC also produce STxs, and infections with some non-O157 EHEC are occasionally associated with the illness caused by O157:H7. A rapid and simple enzyme immunoassay method(EIA) for the detection of STxs was established. This method is based on the use of anti-STx1 or anti-STx2 monoclonal antibody-labelled colloidal gold for detective factor and also used each anti-STx antibody for the capture antibody. The supernatant was used as the test sample after centrifugation of bacterial suspension treated with polymixin B(5,000u/ml). These EIA-tests were specific for all supernatants of EHEC serotypes used, giving positive reactions(more than 1:16-32) by the RPLA method, and permitted the detection of ca.203-812pg of STx1 or STx2 in a 130.MU.l applied to this test. This method will be an extremely useful tool for the detection of STxs from isolates or bacteria on selective agar-plates. (author abst.) |
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