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Accession number;99A0447120
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| Title;Molecular Typing of Methicillin-Resistant Staphylococcus aureus in a University Teaching Hospital. |
| Author;
ISHIMOTO T
(Tokyo Medical And Dental Univ.)
CHIDA T
(Tokyo Medical And Dental Univ.)
OKAMURA N
(Tokyo Medical And Dental Univ.)
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Journal Title;Journal of the Japanese Association for Infectious Diseases
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Journal Code:Z0760A
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ISSN:0387-5911
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VOL.73;NO.3;PAGE.225-232(1999)
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| Figure&Table&Reference;FIG.3, TBL.3 |
| Pub. Country;Japan |
| Language;English |
| Abstract;Plasmid analysis and pulsed-field gel electrophoresis(PFGE) were used to study the epidemiologic relationship among methicillin-resistant Staphylococcus aureus(MRSA) strains isolated at Tokyo Medical and Dental University Hospital. We found that 263 of 276 MRSA isolates had plasmids, which could be classified into 30 different patterns according to the number and plasmid molecular weight. Strains which harboured a single plasmid of approximately 13.4Mds in molecular weight were the most numerous(55.7% of the isolates). These strains were isolated from 14 of 17 hospital wards. The largest number of strains with this plasmid pattern(33 strains) were isolated from a single ward. PFGE typing was then performed to further confirm the relationships among these 33 strains. The PFGE banding patterns of these strains were highly similar. The antibiogram profiles of these strains were also correlated with the PFGE pattern. Thus, the results suggest that these strains are epidemiologically related and spread throughout the ward. Combined plasmid analysis and PFGE were effective fr discriminating the various MRSA isolates. (author abst.) |
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