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Accession number;99A0450019
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| Title;Enzyme Immunoassay for Secretin-like Immunoreactive Substance in Human Plasma. |
| Author;
ITOH H
(Oita Medical Univ.)
NAGANO T
(Oita Medical Univ.)
HAYASHI T
(Oita Medical Univ.)
TAKEYAMA M
(Oita Medical Univ.)
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Journal Title;Japanese Journal of Hospital Pharmacy
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Journal Code:Y0888A
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ISSN:0389-9098
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VOL.25;NO.2;PAGE.162-168(1999)
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| Figure&Table&Reference;FIG.4, REF.16 |
| Pub. Country;Japan |
| Language;English |
| Abstract;A sensitive and specific enzyme immunoassay(EIA) for a secretin-like immunoreactive substance(secretin-IS) was developed using a synthetic carboxy-terminal(C-terminal) fragment(residue 5-27) of a porcine secretin conjugated with .BETA.-D-galactosidase and an anti-rabbit lgG coated imunoplate. The activity of the enzyme on the plate was fluorometrically determined. The present immunoassay allows the detection of 1.7 to 67 fmol/ml(0.068 to 2.7 fmol/well) of secretin. Using the present EIA, the secretin-ISs in human plasma were determined. Moreover, significant changes in the plasma secretin-IS Ievels were found after the oral administration of famotidine(vs. placebo). (author abst.) |
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