Effects of Poly (ADP-ribose) polymerase Inhibitors and NAD on the Apoptosis induced by Actinomycin D in Human Leukemia Cells HL-60.
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Accession number;99A0453849
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| Title;Effects of Poly (ADP-ribose) polymerase Inhibitors and NAD on the Apoptosis induced by Actinomycin D in Human Leukemia Cells HL-60. |
| Author;
OGATA S
(Mie Univ., Mie, Jpn)
NONAKA H
(Mie Univ., Mie, Jpn)
OKUMURA K
(Mie Univ., Mie, Jpn)
TAGUCHI H
(Mie Univ., Mie, Jpn)
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Journal Title;Bull Fac Bioresour Mie Univ
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Journal Code:Y0251B
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ISSN:0915-0471
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VOL.;NO.21;PAGE.73-85(1999)
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| Figure&Table&Reference;FIG.6, REF.26 |
| Pub. Country;Japan |
| Language;English |
| Abstract;We investigated the effects of poly (ADP-ribose) polymerase (PARP) inhibitors and NAD on the apoptosis induced by Actinomycin D in human promyelomonocytic leukemia cells HL-60. As the results, the apoptosis was repressed by various PARP inhibitors especially by 3-aminobenzamide (3-ABA), which is a potent inhibitor for PARP reaction. On the contrary, 3-ABA was no more inhibitory in a cell-free apoptosis system. This result suggests that the inhibitory effect of 3-ABA is effective only before apoptotic cytosol is made up in the cells, i.e., in an early stage of the apoptotic process. But NAD inhibited the apoptosis in the same system depending on NAD concentration. So the inhibition of apoptosis by NAD may be concerned in a later step of apoptosis. (author abst.) |
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