Effect of Cytokines on Production of Insulin-like Growth Factor Binding Proteins (IGFBPs) from Human Fibroblasts in Culture.
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Accession number;99A0391895
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| Title;Effect of Cytokines on Production of Insulin-like Growth Factor Binding Proteins (IGFBPs) from Human Fibroblasts in Culture. |
| Author;
LIU Y
(Tokyo Women's Univ., Tokyo, Jpn)
TSUSHIMA T
(Tokyo Women's Univ., Tokyo, Jpn)
MIYAKAWA M
(Tokyo Women's Univ., Tokyo, Jpn)
ISOZAKI O
(Beijin 514th Hospital, Beijing)
YAMADA H
(Tokyo Women's Univ., Tokyo, Jpn)
XU Z R
(Beijin 514th Hospital, Beijing)
IWAMOTO Y
(Tokyo Women's Univ., Tokyo, Jpn)
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Journal Title;Endocr J
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Journal Code:F0625A
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ISSN:0918-8959
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VOL.46;NO.Supplement;PAGE.S63-S66(1999)
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| Figure&Table&Reference;FIG.3, REF.11 |
| Pub. Country;Japan |
| Language;English |
| Abstract;Bioactivity of Insulin-like growth factors (IGEs) are positively or negatively regulated by IGF binding proteins (IGFBPs). Like IGFs, production of IGFBPs are influenced by a number of hormonal factors. We studied the effects of cytokines on production of IGFBPs in human fibroblasts in culture. Both IL-1.BETA. and TNF-.ALPHA. inhibited IGFBP-3 (42/38 KDa species) production in a concentration dependent manner judged by Western ligand blot. Expression of IGFBP-3 mRNA was also decreased by these cytokines. Moreover, the treatment with IL-1.BETA. and TNF-.ALPHA. resulted in appearance of smaller mol weight (26 KDa) immunoreactive IGFBP-3 fragment(s) which lacked the ability to bind 125I-IGFs, indicating that these cytokines degrade IGFBP-3 via activation of proteases. Both IL-1.BETA. and TNF-.ALPHA. decreased production of IGFBP-4, whereas they increased that of IGFBP-6, IL-6 had little effect on production of IGFBPs. Likewise, interferon-.GAMMA. failed to affect of production of IGFBPs except at high concentrations. The present data demonstrate that cytokines, especially IL-1.BETA. and TNF-.ALPHA. are potent regulators of IGFBPs production and degradation. These cytokines may alter tissue uptake of IGFs and help to counteract the catabolic state induced by them. (author abst.) |
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