Osteo-Anabolic Effects of Human Growth Hormone with 22K- and 20K Daltons on Human Osteoblast-Like Cells.
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Accession number;99A0439788
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| Title;Osteo-Anabolic Effects of Human Growth Hormone with 22K- and 20K Daltons on Human Osteoblast-Like Cells. |
| Author;
WANG D-S
(Tokyo Women's Medical Univ., Tokyo, Jpn)
SATO K
(Tokyo Women's Medical Univ., Tokyo, Jpn)
DEMURA H
(Tokyo Women's Medical Univ., Tokyo, Jpn)
KATO Y
(Tokyo Women's Medical Univ., Tokyo, Jpn)
MARUO N
(Tohso Corp., Kanagawa, Jpn)
MIYACHI Y
(Toho Univ. School Of Medicine, Tokyo, Jpn)
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Journal Title;Endocr J
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Journal Code:F0625A
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ISSN:0918-8959
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VOL.46;NO.1;PAGE.125-132(1999)
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| Figure&Table&Reference;FIG.5, TBL.1, REF.30 |
| Pub. Country;Japan |
| Language;English |
| Abstract;Human growth hormone with 22,000Dal(22K-hGH) stimulates proliferation and differentiation of osteoblasts as well as production of interleukin-6 in vitro and bone formation and remodeling in vivo. To investigate whether hGH isoform with 20Kd(20K-hGH), which accounts for 10% of circulating hGH, elicits similar metabolic effects on skeletal tissues, we studied the biological effects of 20K-hGH in cultured human osteoblast-like cells(HOB). HOB were obtained from trabecular bone explants and cultured in .ALPHA.-MEM supplemented with 10%FCS. In subconfluent cultures, 22K- and 20K-hGH stimulated [3H]thymidine incorporation by 62.+-.27% and 63.+-.23%, respectively(mean.+-.SD, n=8, P>0.1). In confluent cultures, 22K- and 20K-hGH incrased alkaline phosphatase activity by 38.+-.23% and 41.+-.23%(P>0.1), respectively, and increased the osteocalcin concentration in the presence of 10-9M 1,25-(OH)2D3 by 50% and 47%(P<0.1), respectively. Furthermore, both hGHs doubled the interleukin-6(IL-6) concentration in the conditioned medium. RT-PCR analysis revealed that 22K- and 20K-hGH increased IL-6 gene expression 2.2.+-.0.6 and 2.4.+-.0.7-fold, respectively. In summary, we have demonstrated that 20K-hGH elicits equipotent anabolic effects on HOB and stimulates to the same extent the production of IL-6 a cytokine which initiates osteoclastogenesis. These in vitro findings suggest that 22K- and 20K-hGH may equipotently stimulate bone remodeling and elicit anabolic effects on skeletal tissue when administered in vivo. (author abst.) |
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