Stage-Specific Expression of Estrogen Receptor Subtypes and Estrogen Responsive Finger Protein in Preimplantational Mouse Embryos.
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Accession number;99A0439792
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| Title;Stage-Specific Expression of Estrogen Receptor Subtypes and Estrogen Responsive Finger Protein in Preimplantational Mouse Embryos. |
| Author;
HIROI H
(Univ. Tokyo, Tokyo, Jpn)
MOMOEDA M
(Univ. Tokyo, Tokyo, Jpn)
INOUE S
(Saitama Medical School, Saitama, Jpn)
TSUCHIYA F
(Univ. Tokyo, Tokyo, Jpn)
MATSUMI H
(Univ. Tokyo, Tokyo, Jpn)
TSUTSUMI O
(Univ. Tokyo, Tokyo, Jpn)
MURAMATSU M
(Saitama Medical School, Saitama, Jpn)
TAKETANI Y
(Univ. Tokyo, Tokyo, Jpn)
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Journal Title;Endocr J
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Journal Code:F0625A
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ISSN:0918-8959
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VOL.46;NO.1;PAGE.153-158(1999)
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| Figure&Table&Reference;FIG.4, TBL.1, REF.32 |
| Pub. Country;Japan |
| Language;English |
| Abstract;In hope of understanding possible roles of estrogen during early embryogenesis, we examined the expression of both estrogen receptor .ALPHA.(ER.ALPHA.) and ER.BETA., a recently cloned novel subtype, in mouse oocytes and preimplantation embryos by means of reverse transcription polymerase chain reaction(RT-PCR). To investigate whether estrogen actually exerts its action, we further determined the expression of efp(estrogen-responsive finger protein), newly characterized estrogen resposive gene belonging to the RING finger family. ER.ALPHA. mRNA was detected in whole ovaries, cumulus-oocyte complexes, denuded oocytes, 2-cell and 4-cell embryos, whereas it was undertected in 8-cell embroys. Interestingly it reappeared in morulae and blastocysts. ER.BETA. mRNA was detected similarly to ER.ALPHA. except for the absence of ER.BETA. mRNA in morulae. The efp mRNA was detected in whole ovaries, cumulus-oocyte complexes, 4-cell embryos, morulae and blastocysts. The stage specific expression of ER.ALPHA. and ER.BETA. along with detection of the product of the estrogen responsive gene in early preimplantation embryos may indicate the possible physiological roles of estrogen in early embryogenesis. (author abst.) |
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