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Accession number;99A0636878
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| Title;Enzyme Immunoassay of Adrenocorticotropic Hormone-like Immunoreactive Substance in Human Plasma. |
| Author;
NAGANO T
(Oita Medical Univ.)
ITOH H
(Oita Medical Univ.)
SOEDA F
(Oita Medical Univ.)
TAKEYAMA M
(Oita Medical Univ.)
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Journal Title;Japanese Journal of Hospital Pharmacy
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Journal Code:Y0888A
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ISSN:0389-9098
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VOL.25;NO.3;PAGE.257-263(1999)
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| Figure&Table&Reference;FIG.3, TBL.2, REF.12 |
| Pub. Country;Japan |
| Language;English |
| Abstract;A sensitive and specific double-antibody enzyme immunoassay(EIA) for adrenocorticotropic hormone-like immunoreactive substances(ACTH-ISs) was developed. In competitive reactions, the ACTH(1-24)-antibody was incubated with both ACTH(1-24) standard(or sample) and .BETA.-D-galactosidase labeled synthetic human ACTH(1-24)(delayed addition). The free and antibody-bound enzyme haptens were separated by using an anti-rabbit IgG coated immunoplate. The activity of the enzyme on the plate was fluorometrically determined. The present immunoassay allows for the detection of 2 to 600fmol/mL of ACTH(1-24). Using the proposed EIA, ACTH(1-24)-ISs in human plasma were thus determined. As a result, the levels of ACTH(1-24)-ISs in human plasma were found to be about 3.7fmol/mL. (author abst.) |
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