Science Links Japan | The Effect of Epidermal Growth Factor on the Preimplantation Development, Implantation and Its Receptor Expression in Mouse Embryos.

The Effect of Epidermal Growth Factor on the Preimplantation Development, Implantation and Its Receptor Expression in Mouse Embryos.

Accession number;99A0427207

Title;The Effect of Epidermal Growth Factor on the Preimplantation Development, Implantation and Its Receptor Expression in Mouse Embryos.

Author; KIM C-H (Univ. Ulsan, Seoul, Kor) CHAE H-D (Univ. Ulsan, Seoul, Kor) CHEON Y-P (Univ. Ulsan, Seoul, Kor) KANG B-M (Univ. Ulsan, Seoul, Kor) CHANG Y-S (Univ. Ulsan, Seoul, Kor) MOK J-E (Univ. Ulsan, Seoul, Kor)

Journal Title;J Obstet Gynaecol Res

Journal Code:Y0696A

ISSN:1341-8076

VOL.25;NO.2;PAGE.87-93(1999)

Figure&Table&Reference;FIG.3, TBL.2, REF.29

Pub. Country;Japan

Language;English

Abstract;Objective: To investigate the influence of epidermal growth factor(EGF) on preimplantation development, implantation, and expression of epidermal growth factor receptor(EGFR) itself in mouse embryos. Materials and Method: Eight-cell stage mouse embryos were cultured for 48 hours with EGF at concentrations of 0.1, 1.0, 10 and 100ng/ml. Embryos not treated with EGF were served as control. The percentages of embryos which developed to the expanded, hatched blastocyst stage and in vitro implantation at 48 hours were determined. Reverse transcription-polymerase chain reaction(RT-PCR) has been used to examine the expression of EGFR in developed hatched blastocysts. Following reverse transcription, strategically designed nested primers, optimized for specificity, were used for amplification from the cDNA equivalent of a single embryo, The products were then verified by restriction enzyme digestion and sequence analysis. Restults were analyzed with x2 test and Student's t-test as apropriate, and statistical significance was defined as p<0.05. Results: The percentages of fully expanded blastocysts at 48 hours in all the EGF treated group were not significantly different from the control. The percentages of hatched blastocysts were significantly higher in the EGF treatment group at 0.1ng/ml (90.5.+-.9.8%) compared to the control (82.1.+-.7.2%), 1.0ng/ml (82.2.+-.12.7%), and 100ng/ml (81.9.+-.11.8%) (p<0.05, p<0.05, p<0.05, respectively). The percentages of hatched blastocysts were significantly higher in the EGF treatment group at 10ng/ml (89.4.+-.7.5%) compared to the control, and 100ng/ml (p<0.05, p<0.05, respectively). The percentages of attached blastocysts in vitro were significantly higher following incubation with EGF at concentrations of 0.1ng/ml (37.0.+-.17.0%), 1.0ng/ml (32.0.+-.14.3%), 10ng/ml (21.3.+-.7.2%) compared to the control (9.5.+-.7.7%) (p<0.05, p<0.05, p<0.05, respectively).... (author abst.)

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