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Accession number;99A0465597
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| Title;Functional Changes of Cultured Endothelial Cells Induced by High Ambient D-Glucose: Molecular Biological Analysis of ICAM-1 Expression. |
| Author;
OTSUKA MASAKI
(Tokai Univ., Sch. of Med.)
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Journal Title;Journal of the Japan Diabetic Society
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Journal Code:Z0279B
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ISSN:0021-437X
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VOL.42;NO.3;PAGE.193-200(1999)
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| Figure&Table&Reference;FIG.5, TBL.1, REF.29 |
| Pub. Country;Japan |
| Language;Japanese |
| Abstract;Atherosclerotic changes in human arteries demonstrate expression of the inter cellular adhesion molecule-1(ICAM-1) on endothelial cells and the subsequent attachment of monocytes. The effects of high concentrations of D-glucose on the expression of ICAM-1 by cultured human endothelial cells were examined by immunofluorescence microscopy and reverse transcription polymerase chain reaction(RT-PCR) analysis. Immunofluorescence staining revealed that cultured endothelial cells in the presence of 30mM of D-glucose resulted in an increase in ICAM-1 expression, relative to that apparent for cells culutred at 5mM D-glucose. Similarly, RT-PCR showed that exposure of cells to 30mM D-glucose resulted in a significant increase in the amount of ICAM-1 mRNA (P<0.03). The antioxidant N-acetyl-L-cysteine(NAC) at a concentration of 10mM reduced the abundance of nuclear factor .KAPPA.B(NF-.KAPPA.B) and ICAM-1 mRNA in cells cultured in fibronectin-coated dishes, but had no effect on laminin-coated dishes. Furthermore, high concentrations of glucose (30mM) increased the amounts of ICAM-1 and NF-.KAPPA.B mRNAs in cells cultured on either fibronectin or laminin. These results suggest that the nature of the extracellular matrix can influence NF-.KAPPA.B signaling in the endothelial cells and that high concentrations of D-glucose increase the expression of both ICAM-1 and NF-.KAPPA.B. (author abst.) |
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