Genetic Diagnosis of Prostate Cancer-Clinical Significance of Chromosomal Deletions by FISH Technique.
|
Accession number;99A0415042
|
| Title;Genetic Diagnosis of Prostate Cancer-Clinical Significance of Chromosomal Deletions by FISH Technique. |
| Author;
MATSUYAMA HIDEYASU
(Yamaguchi Red Cross Hosp.)
YOSHIHIRO SATORU
(Yamaguchi Univ., Sch. of Med.)
NAITO KATSUSUKE
(Yamaguchi Univ., Sch. of Med.)
PAN Y
(Karolinska Hospital, Stockholm, Swe)
TRIBUKAIT B
(Karolinska Hospital, Stockholm, Swe)
EKMAN P
(Karolinska Hospital, Stockholm, Swe)
BERGERHEIM U S R
(Karolinska Hospital, Stockholm, Swe)
|
Journal Title;Nishinihon Journal of Urology
|
Journal Code:Z0253B
|
ISSN:0029-0726
|
|
VOL.61;NO.3;PAGE.209-216(1999)
|
| Figure&Table&Reference;FIG.6, TBL.5, REF.21 |
| Pub. Country;Japan |
| Language;Japanese |
| Abstract;In spite of the general agreement that cancer is the disease of gene, little is known about the genetic background regarding oncogenesis and disease progression in prostate cancer. Deletions of chromosomes 8p, 10q and 16q frequently occur in prostate cancer, while the role of these chromosomal deletions has not yet been clarified. Thus, the FISH technique was applied to detect these deletions, and a comparison was made with clinical parameters including tumor differentiation, grade and stage in 53 cases of prostate cancer. Both the region-specific cosmid probes for 8p22, 8p23-pter, 10q24-qter, 16q22-qter and the corresponding centromere probes were co-hybridized, respectively, and detected by the dual-color FISH technique. Forty-five case(14 pT2, 10 pT3 and 21 pN1 tumors) were further evaluated regarding the disease progression of individual cases, with a median follow-up period of 53 months. In total, 74% of chromosome 8p, in particular 66% of 8p22, were deleted. The overall frequencies of deletions for 10q and 16q were both 55%. The 8p and 16q deletions were significantly correlated to tumor de-differentiation(p<0.01 and <0.05, respectively), while 10q deletions were not. Patients with 8p22 deletions had a significantly shorter progression-free survival than those who retained 8p22(Log-rank test, P<0.05). A multivariate analysis by Cox's hazard proportional model showed 8p22 deletion to be an independent prognostic indicator for disease progression together with tumor stage. We concluded that an estimation of 8p22 deletion may serve as a prognostic marker for disease progression in prostate cancer. (author abst.) |
|
|
|
Related Articles;
|
|
