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Accession number;99A0800274
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| Title;Evaluation of an Automatic Device to Prepare Fibronectin Eyedrops Using a Patient's Own Plasma. |
| Author;
YOSHIDA H
(Ymaguchi Univ.)
TSUBONE K
(Ymaguchi Univ.)
YAMASHITA R
(Ymaguchi Univ.)
OHTSUBO Y
(Ymaguchi Univ.)
ISHIMITSU T
(Ymaguchi Univ.)
KAMIYA A
(Ymaguchi Univ.)
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Journal Title;Japanese Journal of Hospital Pharmacy
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Journal Code:Y0888A
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ISSN:0389-9098
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VOL.25;NO.4;PAGE.361-367(1999)
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| Figure&Table&Reference;FIG.5, TBL.2, REF.12 |
| Pub. Country;Japan |
| Language;English |
| Abstract;Fibronectin(FN) has been reported to be effective for the treatment of corneal trophic ulcers, but the FN eyedrop preparation is not a simple technique. We previously developed an automatic device to prepare FN eyedrops using a patient's own plasma (Yoshida et al. Jpn. J. Hosp. Pharm., 24, 493-498 (1998)). The aim of this study was to evaluate the efficiency of this automatic device. FN eyedrops were prepared using plasma from healthy individuals either with an automatic device or a manual process using gelatin-coupled Sepharose 4B(GS4B) and Sephadex G-25 (PD-10). The average FN concentration and the recovery rate in the eyedrops prepared using the automatic device were 653.+-.57.MU.g per ml and 55.+-.5%, respectively, but those prepared using the manual process were 563.+-.50.MU.g per ml and 46.+-.6%, respectively. The operation of the device was easy and the recovery values of FN were similar among all operators. The total preparation time of FN eyedrops was approximately 1 hour. This automatic device was thus found to be useful for preparing FN eyedrops from the patient's own plasma. (author abst.) |
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