Callus Formation and Plantlet Regeneration through In vitro Culture of Immature Embryo and Seedling in Chinese Chive (Allium tuberosum Rottler).
|
Accession number;99A0922087
|
| Title;Callus Formation and Plantlet Regeneration through In vitro Culture of Immature Embryo and Seedling in Chinese Chive (Allium tuberosum Rottler). |
| Author;
XUE H-M
(Suntory Res. Center, Inst. for Fundam. Res.)
ARAKI HAJIME
(Niigata Univ., Univ. Farm)
KANAZAWA TOSHINARI
(Iwate Univ., Fac. of Educ.)
HARADA TAKASHI
(Hokkaido Univ., Fac. of Agric.)
YAKUWA TOSHIRO
(Hokkaido Univ., Fac. of Agric.)
|
Journal Title;Journal of the Japanese Society for Horticultural Science
|
Journal Code:F0626A
|
ISSN:0013-7626
|
|
VOL.66;NO.2;PAGE.353-358(1997)
|
| Figure&Table&Reference;FIG.2, TBL.4, REF.15 |
| Pub. Country;Japan |
| Language;Japanese |
| Abstract;This study was carried out to establish an efficient method for propagating Chinese chive (Allium tuberosum Rottler) through callus culture. Compact and white callus was induced from seedlings on AZ medium supplemented with 10.MU.M 2,4-dichlorophenoxyacetic acid(2,4-D), 1 to 10.MU.M 2,4-D and 0.01 to 0.1.MU.M 6-benzyladenine(BA), and from 3-4mm immature embryos cultures on 1 to 10.MU.M 2,4-D and 0 to 10.MU.M BA. Seedling-derived callus proliferated when subcultured on Murashige and Skoog(MS) medium containing 1.MU.M 2,4-D. Higher rates of the multiple shoot formation were obtained from these proliferated calli on MS medium supplemented with 25.MU.M BA with or without .ALPHA.-naphthaleneacetic acid(NAA). Multiple shoot growth and plant regeneration rate were accelerated when shoots were transferred to a medium without plant growth regulators. Regenerated plantlets developed vigorously after being successfully acclimated in soil. (author abst.) |
|
|
|
Related Articles;
|
|
|
