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Accession number;00A0163468
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| Title;Photo Cross-Linking Reagent for DNA Immobilization and DNA Biosensor Applications. |
| Author;
NAKANO KOJI
(Kyushu Univ., Grad. Sch.)
SAI KEISUKE
(Kyushu Univ., Grad. Sch.)
TAGUCHI SHIN'YA
(Kyushu Univ., Grad. Sch.)
MAEDA MIZUO
(Kyushu Univ., Grad. Sch.)
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Journal Title;Abstracts. Symposium on Biofunctional Chemistry
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Journal Code:L0836A
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ISSN:
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VOL.14th;NO.;PAGE.16-17(1999)
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| Figure&Table&Reference; |
| Pub. Country;Japan |
| Language;Japanese |
| Abstract;An amino derivative of 4,5',8-trimethylpsoralen (Pso-NH2) was newly synthesized and applied to DNA immobilization on gold surfaces in a combined use of SAMs from 3,3'-dithio-bis(propionic acid-N-hydroxysuccinimide ester) (DTSP). The proposed method is as follows: a base DTSP submonolayer was bound to a gold substrate via chemisorption. The base layer was first reacted with Pso-NH2, and the resulting Pso-NH2 submonolayer was then reacted with DNA to form inter-strand cross-links with the pyrimidine bases in the DNA through photocycloaddition. The ir spectrum of the DNA/Pso-NH2/Au structure in reflection-absorption mode showed absorption bands which were typical for DNA while no clear peak was observed for the Pso-NH2/Au structure except for C=O stretches. Cyclic voltammograms for the DNA-modified gold electrode with the DNA/Pso-NH2/Au structure measured in phosphate buffer solution showed a clear oxidation peak at around +950mV (Ag/AgCl). This peak was attributed to the oxidation of the purine bases in the DNA immobilized on the electrode surface. We concluded that the ad layer formation through Pso-NH2 should give a versatile menas in modifying solid surfaces with DNA. (author abst.) |
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