Science Links Japan | In vitro gene transfer using N-(3-dimethylaminopropyl)acrylamide-co-acrylamide random copolymers with various unit compositions.

In vitro gene transfer using N-(3-dimethylaminopropyl)acrylamide-co-acrylamide random copolymers with various unit compositions.

Accession number;00A0163469

Title;In vitro gene transfer using N-(3-dimethylaminopropyl)acrylamide-co-acrylamide random copolymers with various unit compositions.

Author; YAMAOKA TETSUJI (Kyoto Inst. of Technol., Fac. of Text. Sci.) YAMADA SHUSAKU (Kyoto Inst. of Technol., Fac. of Text. Sci.) IWASE REIKO (Kyoto Inst. of Technol., Fac. of Text. Sci.) MURAKAMI AKIRA (Kyoto Inst. of Technol., Fac. of Text. Sci.)

Journal Title;Abstracts. Symposium on Biofunctional Chemistry

Journal Code:L0836A

ISSN:

VOL.14th;NO.;PAGE.18-19(1999)

Figure&Table&Reference;

Pub. Country;Japan

Language;Japanese

Abstract;To date we figured out the two key factors in the chemical structures of the polycations which lead high expression of introduced gene in vitro. One is the tertiary or quaternary amino groups and the other is the non-charged hydrophilic groups such as hydroxyl groups. In the present paper, the expression of the transferred gene by the osmotic shock procedure using N-(3-dimethylaminopropyl)acrylamide-co-acrylamide random copolymers(DMAPAA-AAm) with various unit compositions was studied. The percent gene expression and the amount of the expressed protein in the cells were greatly affected by the unit composition of the copolymers. Copolymers with the cationic composition of 30-40% showed the highest expression. The amide groups of the polycations were found to be able to impart the hydrophilicity to the formed polycation/DNA complexes as well as the hydroxyl groups and this hydrophilicity plays an important role in the step of polycation/DNA recognition by the transcription factors. (author abst.)

Related Articles;