Regulation of Gene Expression using Psoralen-conjugated Oligo(nucleoside phosphorothioate)s and Analysis for the Mechanism of Photodynamic Antisense Regulation.
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Accession number;00A0163470
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| Title;Regulation of Gene Expression using Psoralen-conjugated Oligo(nucleoside phosphorothioate)s and Analysis for the Mechanism of Photodynamic Antisense Regulation. |
| Author;
IWASE REIKO
(Kyoto Inst. of Technol., Fac. of Text. Sci.)
YAMAYOSHI ASAKO
(Kyoto Inst. of Technol., Fac. of Text. Sci.)
NISHIDA JUN'ICHI
(Med. Inst. of Bioregulation, Kyushu Univ.)
YAMAOKA TETSUJI
(Kyoto Inst. of Technol., Fac. of Text. Sci.)
WAKE NORIO
(Med. Inst. of Bioregulation, Kyushu Univ.)
MURAKAMI AKIRA
(Kyoto Inst. of Technol., Fac. of Text. Sci.)
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Journal Title;Abstracts. Symposium on Biofunctional Chemistry
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Journal Code:L0836A
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ISSN:
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VOL.14th;NO.;PAGE.20-21(1999)
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| Figure&Table&Reference; |
| Pub. Country;Japan |
| Language;Japanese |
| Abstract;To enhance the antisense regulatory effect of oligo(nucleoside phosphorothioate)s(S-Oligo), a photo-crosslinking reagent, 4,5',8-trimethypsoralen, was used in this study. Without UVA-irradiation, psoralen-conjugated oligo(nucleoside phosphorothioate)s(Ps-S-Oligo) did not show any inhibitory effects on the proliferation of cervical carcinoma cells. On the other hand, Ps-S-Oligo complementary to HPV18 mRNA did appreciably inhibit the proliferation of C4II cells (HPV18 positive) with UVA-irradiation. In conclusion, Ps-S-Oligo could inhibit the cellular proliferation in sequence and tissue specific manners with UVA-irradiation, and photodynamic antisense regulation may be one of the promising methods for gene therapy. (author abst.) |
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