Design of modified oligonucleotides for the photo-regulation of gene expression. (3). Photo-regulation of duplex and triplex forming activity and its application.
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Accession number;00A0163472
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| Title;Design of modified oligonucleotides for the photo-regulation of gene expression. (3). Photo-regulation of duplex and triplex forming activity and its application. |
| Author;
ASANUMA HIROYUKI
(Univ. of Tokyo, Grad. Sch.)
LIANG X
(Univ. of Tokyo, Grad. Sch.)
YOSHIDA TAKAYUKI
(Univ. of Tokyo, Grad. Sch.)
ITO TAKANORI
(Univ. of Tokyo, Grad. Sch.)
YAMAZAWA AKIRA
(Univ. of Tokyo, Grad. Sch.)
KOMIYAMA MAKOTO
(Univ. of Tokyo, Grad. Sch.)
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Journal Title;Abstracts. Symposium on Biofunctional Chemistry
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Journal Code:L0836A
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ISSN:
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VOL.14th;NO.;PAGE.24-25(1999)
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| Figure&Table&Reference; |
| Pub. Country;Japan |
| Language;Japanese |
| Abstract;Our purpose is to regulate the gene expression by photo-stimulus. For this purpose, azobenzene residue has been introduced into the side chain of the oligonucleotide. In the present paper, the duplex- and triplex-forming activities of oligonucleotide are investigated by using these modified oligonucleotides. When the azobenzene in the oligonucleotides was isomerized from the trans-form to the cis-form by photo-irradiation (300nm<.LAMBDA.<400nm), the melting temperatures of the duplex and triplex between the oligonucleotides and their complementary counterparts were significantly lowered. On irradiating visible light (.LAMBDA.>400nm), however, the Tms were elevated and became virtually the same as the values before the UV irradiation. Thus, the duplex and triplex were formed and dissociated without changing pH and ionic strength. We have also applied these modified oligonucleotides to the photo-regulation of enzymatic reaction. (author abst.) |
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