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Accession number;00A0163483
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| Title;Peroxidase Activity of .BETA..ALPHA..BETA..ALPHA.-Polypeptide Containing Iron-porphyrin and Arginine. |
| Author;
NISHINO HIDEKAZU
(Kyushu Inst. of Technol., Fac. of Eng.)
TOMIZAKI KIN'YA
(Kyushu Inst. of Technol., Fac. of Eng.)
NISHINO NORIKAZU
(Kyushu Inst. of Technol., Fac. of Eng.)
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Journal Title;Abstracts. Symposium on Biofunctional Chemistry
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Journal Code:L0836A
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ISSN:
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VOL.14th;NO.;PAGE.48-49(1999)
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| Figure&Table&Reference; |
| Pub. Country;Japan |
| Language;Japanese |
| Abstract;Cytochrome c peroxidase which catalyzes the oxidation of a substrate has one heme and two histidine residues (distal His and proximal His) at its active site. The distal His could serve as a proton donor and cooperate with charged arginine(Arg) to make the distal side substantially polar (pull effect). The proximal imidazolate ligand of His could serve as a strong internal electron donor to destabilize the O-O bond of the hydroperoxoiron(III)-porphyrin (push effect). In order to develop the de novo designed polypeptide in enzymatic activity, we attempted to engineer the iron(III)-porphyrin linked .BETA..ALPHA..BETA..ALPHA.-polypeptide by attachment of positive charged Arg to its active site. (author abst.) |
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