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Accession number;00A0163497
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| Title;Salt induced GG-selective electron transfer: switching of the mechanism of DNA photocleavage by p-nitrobenzamide derivatives. |
| Author;
NINOMIYA KEIKO
(Univ. of Tokyo, Grad. Sch.)
SUGIYAMA TOORU
(Univ. of Tokyo, Grad. Sch.)
KURODA REIKO
(Univ. of Tokyo, Grad. Sch.)
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Journal Title;Abstracts. Symposium on Biofunctional Chemistry
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Journal Code:L0836A
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ISSN:
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VOL.14th;NO.;PAGE.78-79(1999)
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| Figure&Table&Reference; |
| Pub. Country;Japan |
| Language;Japanese |
| Abstract;The UV-reactive p-nitrobenzamide(pNoBz) derivative has been shown to possess two different mechanisms of photocleavage depending on the r-value, i.e. the known hydrogen abstraction from sugar moiety and the newly found electron transfer from guanine base. On usual conditions (10-50mM Na cacodylate buffer, pH7.0), the DNA photocleavage reaction by pNoBz derivative proceeded primarily by hydrogen abstraction. Photocleavage reaction with various salt concentrations (75-750mM NaCl), have shown that it cleave 5'-AAATA-3' and 5'-GG-3' at lower and higher salt concentrations, respectively. Thus the electron transfer was accelerated and at the same time hydrogen abstraction was decelerated with increasing concentration of salt. (author abst.) |
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