Effect of seed size on the tissue culture response of callus from mature embryos of wheat species.

Accession number;00A0068166
Title;Effect of seed size on the tissue culture response of callus from mature embryos of wheat species.
Author; SAYAR M T (Bogazici Univ., Istanbul, Tur) BIRSIN M A (Univ. Ankara, Ankara, Tur) ULUKAN H (Univ. Ankara, Ankara, Tur) OEZGEN M (Univ. Ankara, Ankara, Tur)
Journal Title;Wheat Inf Serv
Journal Code:Y0174A
ISSN:0510-3517
VOL.;NO.89;PAGE.1-6(1999)
Figure&Table&Reference;TBL.1, REF.27
Pub. Country;Japan
Language;English
Abstract;Effect of seed size on the culture response of callus from mature embryos of different genotypes of diploid (T. monococcum L. and T. boeoticum L.), tetraploid (T. durum Desf. 'Kunduru-1149') and hexaploid (T. aestivum L. 'Bezostaja-1') wheat was investigated using recently developed endosperm-supported mature embryo culture technique. After the separation of seeds among each genotype as being large and small, they are imbibed and wounded by moving the embryos from the seed without excision completely. Following their incubation in 8mg/l 2,4-dichlorophenoxyacetic acid (2,4-D) solution for callus induction, they are transferred onto auxin-free Murashige-Skoog (MS) medium for regeneration. Callus induction frequency, fresh weight of callus, regeneration capacity of callus and culture efficiency were found significantly higher in large seeds compare to small seeds in all genotypes. There were also significant correlation between seed weight and callus weight (r=0.86**) as well as callus weight and regenerative callus number (r=0.85**). These results suggested that effircient regenerative callus induction from endosperm-supported mature embryo culture of wheat could be obtained by using large seeds. Therefore, this technique provides an alternative use of wheat mature embryos as an explant which unlike immature embryos, are available at every periods of each year in unlimited quantities. (author abst.)
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