Local expression of cytokine mRNA in a rat Escherichia coli epididymitis model and cultured epididymal cells.
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Accession number;00A0221178
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| Title;Local expression of cytokine mRNA in a rat Escherichia coli epididymitis model and cultured epididymal cells. |
| Author;
TANAKA KAZUSHI
(Kobe Seikojo Shinko Byoin)
FUJISAWA MASATO
(Kobe Univ.)
ARAKAWA SOICHI
(Kobe Univ.)
KAMIDONO SADAO
(Kobe Univ.)
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Journal Title;Japanese Journal of Chemotherapy
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Journal Code:F0608A
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ISSN:1340-7007
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VOL.48;NO.1;PAGE.1-6(2000)
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| Figure&Table&Reference;FIG.5, REF.32 |
| Pub. Country;Japan |
| Language;Japanese |
| Abstract;In a rat model of Escherichia coli epididymitis, Northern blot analysis and reverse transcription-polymerase chain reaction(RT-PCR) demonstrated that expression of IL-6 mRNA in the epididymis increased, reached a maximum as early as 6h after bacterial challenge, and then decreased. No signals of expression detected on the contralateral side or in the controls. Expression of other cytokines (IL-1.BETA., IL-2, TNF-.ALPHA., IFN-.GAMMA.) was also determined by RT-PCR. After bacterial challenge, IL-.BETA. mRNA was detected in the epididymis as well as IL-6 mRNA. The TNF-.ALPHA. mRNA signal, but not of IFN-.GAMMA. mRNA, increased greatly after bacterial challenge, although both TNF-.ALPHA. and IFN-.GAMMA. mRNAs were detected in uninfected epididymis. Histopathological examination showed no apparent changes at 6h after infection, and only mild interstitial cellular infiltration at 24h. Immunohistochemical examination showed that epithelial cells produced IL-6. Cultured epithelial cells also expressed IL-6. IL-1.BETA., and TNF-.ALPHA. mRNA after lipopolysaccharide stimulation. The results suggest that mRNAs for specific cytokines are expressed locally as early as 6h in response to bacterial genital infection before the histopathological changes. This rapid expression may be important in host defense against local genital infection. (author abst.) |
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