Antiviral Effect of (S)-1-(3-hydroxy-2-phosphonylmethoxypropyl) cytosine on Adenovirus.
|
Accession number;00A0262552
|
| Title;Antiviral Effect of (S)-1-(3-hydroxy-2-phosphonylmethoxypropyl) cytosine on Adenovirus. |
| Author;
NAKAJIMA HARUHIKO
(Mitsubishi Kagaku Bio-Clinical Lab., Inc.)
GOTO MASAKI
(Mitsubishi Kagaku Bio-Clinical Lab., Inc.)
SHIMADA YUKIKO
(Mitsubishi Kagaku Bio-Clinical Lab., Inc.)
ISHIKO HIROAKI
(Mitsubishi Kagaku Bio-Clinical Lab., Inc.)
INAGAWA WAKA
(Yokohama City Univ., Sch. of Med.)
ITO NORIHIKO
(Yokohama City Univ., Sch. of Med.)
UCHIO EIICHI
(Yokohama City Univ., Sch. of Med.)
ONO SHIGEAKI
(Yokohama City Univ., Sch. of Med.)
AOKI KOKI
(Aoki Ganka)
|
Journal Title;Journal of Japanese Ophthalmological Society
|
Journal Code:Z0666A
|
ISSN:0029-0203
|
|
VOL.104;NO.2;PAGE.77-81(2000)
|
| Figure&Table&Reference;FIG.2, TBL.2, REF.16 |
| Pub. Country;Japan |
| Language;Japanese |
| Abstract;Purpose: Adenovirus is the most frequent causative virus of conjunctivitis in Japan. Recently (S)-1-(3-hydroxy-2-phosphonylmethoxypropyl) cytosine (HPMPC) has been promoted as a new drug against adenoviral conjunctivitis. So we examined the antiviral activity of HPMPC against adenoviruses in vitro. Method: The antiviral activity of HPMPC against adenovirus (Ad) type 3, type 4, type 19, and type 37 isolated from conjunctivial scrapings in Japan and the prototype of adenovirus type 5 was examined by plaque reduction assay using A 549 cells in vitro. Results: The 50% inhibitory dose (ID50) of HPMPC was 3.50 (1.44-4.79) .MU.g/ml for Ad type 3, 4.50 (4.17-4.92) .MU.g/ml for Ad type 4, 2.11 (1.03-3.13) .MU.g/ml for Ad type 5, 1.64 (1.40-2.02) .MU.g/ml for Ad type 19, and 2.02 (1.17-2.73) .MU.g/ml for type 37. The 50% cytotoxic dose of HPMPC for A 549 cells was 205 .MU.g/ml by the deoxythimidine uptake inhibition test, and 537 .MU.g/ml by the trypan blue exclusion inhibition test. Conclusions: HPMPC proved to be highly effective in inhibiting replication of adenoviruses at lower concentrations than the cytotoxic level in vitro. (author abst.) |
|
|
|
Related Articles;
|
|
|
