|
Accession number;00A0262554
|
| Title;Effects of Corticosteroid on Porcine Retinal Pigment Epithelial Cells in Culture. 2. Effects on Phagocytosis and Lysosomal Activity. |
| Author;
KAWAHARA SUMIE
(Kansai Med. Univ.)
KISHIMOTO NAOKO
(Kansai Med. Univ.)
SUGASAWA KEIJI
(Kansai Med. Univ.)
UYAMA MASANOBU
(Kansai Med. Univ.)
|
Journal Title;Journal of Japanese Ophthalmological Society
|
Journal Code:Z0666A
|
ISSN:0029-0203
|
|
VOL.104;NO.2;PAGE.86-90(2000)
|
| Figure&Table&Reference;FIG.5, REF.19 |
| Pub. Country;Japan |
| Language;Japanese |
| Abstract;Purpose: The effects of a corticosteoid on the phagocytosis and lysosomal activity of cultured porcine retinal pigment epithelium (RPE) cells were investigated. Methods: After exposing cultured RPE cells to various concentrations (10, 50, 100, 500, 1,000 nM) of betamethasone sodium phosphate (betamethasone), the cells were incubated with latex microspheres for 6 hours. Results: The number of latex microspheres phagocytized by the cultured RPE cells was inhibited by 50 nM betamethasone within 24 hours. Ten-nM betamethasone did not inhibit the proliferation of eultured RPE cells, but ingestion of latex microspheres by the cells was inhibited after 3 days. Lysosomal activity (acid phosphatase, .BETA.-glucuronidase) of RPE cells was inhibited by a high concentration (500 nM) of betametasone. Conclusion: These results suggest that corticosteroid inhibits the phagocytosis and lysosomal activity of cultured RPE cells. (author abst.) |
|
|
|
Related Articles;
|
|
