Science Links Japan | Determining S-genotypes of Two Sweet Cherry(Prunus avium L.) Cultivars, 'Takasago(Rockport Bigarreau)' and 'Hinode(Early Purple)'.

Determining S-genotypes of Two Sweet Cherry(Prunus avium L.) Cultivars, 'Takasago(Rockport Bigarreau)' and 'Hinode(Early Purple)'.

Accession number;00A0234047

Title;Determining S-genotypes of Two Sweet Cherry(Prunus avium L.) Cultivars, 'Takasago(Rockport Bigarreau)' and 'Hinode(Early Purple)'.

Author; YAMANE H (Kyoto Univ., Kyoto) TAO R (Kyoto Univ., Kyoto) MURAYAMA H (Yamagata Univ., Tsuruoka) ISHIGURO M (Yamagata Prefectural Horticultural Experiment Station, Sagae) ABE Y (Yamagata Prefectural Horticultural Experiment Station, Sagae) SOEJIMA J (National Inst. Fruit Tree Sci., Morioka) SUGIURA A (Kyoto Univ., Kyoto)

Journal Title;Journal of the Japanese Society for Horticultural Science

Journal Code:F0626A

ISSN:0013-7626

VOL.69;NO.1;PAGE.29-34(2000)

Figure&Table&Reference;FIG.3, TBL.2, REF.24

Pub. Country;Japan

Language;English

Abstract;'Takasago(Rockport Bigarreau)', one of the major early ripening cultivars of sweet cherry in Japan, was reported to be cross-incompatible with 'Hinode(Early Purple)'. Thus, they were assumed to have the same S-alleles, although their S-genotypes were unknown. However, when 'Takasago' was cross-pollinated with 'Hinode' at the Yamagata Prefectural Horicultural Experiment Station, the fruit set normally(unpublished data), which suggests that they are cross-compatible. In this study, we re-evaluated crossability between the two cultivars by controlled pollination tests and determined their S-genotypes by the molecular typing system recently developed for S-alleles of sweet cherry. The cross-pollination tests conducted at two different locations in Japan showed that 'Takasago' and 'Hinode' were self-incompatible but reciprocally cross-compatible. Furthermore, 2D-PAGE(two dimensional-polyacrylamide gel electro-phoresis) analysis for stylar extracts revealed that they have none of the common S-RNases, S-gene products in styles of sweet cherry, which suggests that their S-genotypes are completely different. By S-RNase spot patterns in 2D-PAGE profiles and RFLP(restriction fragment length polymorphism) analysis, S-genotypes of 'Takasago' and 'Hinode' were proposed to be S1 S6 and S2 Sx(Sx is a novel S-allele different from S1 to S6), respectively. This implies that 'Hinode' is cross-compatible with all cultivars of known S-genotypes. The usefulness of biochemical and molecular methods for S-allele typing employed in this study discussed. (author abst.)

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