Polaprezinc, a gastroprotective agent. Attenuation of monochloramineevoked gastric DNA fragmentation.
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Accession number;00A0234926
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| Title;Polaprezinc, a gastroprotective agent. Attenuation of monochloramineevoked gastric DNA fragmentation. |
| Author;
SUZUKI H
(Keio Univ., Tokyo, Jpn)
MORI M
(Keio Univ., Tokyo, Jpn)
SETO K
(Zeria Pharmaceutical, Saitama, Jpn)
NAGAHASHI S
(Keio Univ., Tokyo, Jpn)
MORITA H
(Zeria Pharmaceutical, Saitama, Jpn)
SUZUKI M
(National Tokyo Medical Center, Tokyo, Jpn)
MIURA S
(National Defense Medical Coll., Tokorozawa, Jpn)
YONETA T
(Zeria Pharmaceutical, Saitama, Jpn)
ISHII H
(Keio Univ., Tokyo, Jpn)
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Journal Title;J Gastroenterol
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Journal Code:Z0748A
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ISSN:0944-1174
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VOL.34;NO.Supplement 11;PAGE.43-46(1999)
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| Figure&Table&Reference;FIG.2, REF.18 |
| Pub. Country;Japan |
| Language;English |
| Abstract;We previously reported that NH2Cl induced extensive DNA fragmentation in gastric cells. Polaprezinc, a zinc-carnosine chelate compound, is reported to be a potent antioxidant in gastric mucosa. The present study was designed to examine whether polaprezinc could attenuate the NH2Cl-induced DNA damage. Gastric cell lines, MKN45, were exposed to NH2Cl in Ca2+-containing Hanks' balanced salt solution. DNA fragmentation was evaluated by photometric enzyme immunoassay for in vitro determination of cytoplasmic mono- and oligonucleosomes. Polaprezinc, L-carnosine, and zinc sulfate (ZnSO4) were added to the cell incubation medium to evaluate the inhibitory effect on the formation of cytoplasmic mono- and oligonu-cleosomes. Separately, the bleaching level of .BETA.-carotene with the addition of each test solution was evaluated to confirm the inhibitory effect against hypochlorous acid. Polaprezinc or L-carnosine, but not ZnSO4, at a concentration of 0.001 mM, significantly attenuated the increased levels of cytoplasmic mono- and oligonucleo-somes evoked by 0.001 mM NH2Cl. Polaprezinc and L-carnosine, but not ZnSO4, also inhibited NH2Cl-induced .BETA.-carotene bleaching in the cell-free system. In conclusion, polaprezinc, especially its subportion L-carnosine, inhibited NH2Cl-evoked gastric epithelial DNA fragmentation, suggesting a role for this agent in preventing the progression of gastric epithelial injury induced by NH2Cl. (author abst.) |
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