Identification of a Free Non-tryptophan Fluorophore in Water-soluble Fraction of Human Brunescent Cataractous Lens Nucleus.
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Accession number;00A0446396
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| Title;Identification of a Free Non-tryptophan Fluorophore in Water-soluble Fraction of Human Brunescent Cataractous Lens Nucleus. |
| Author;
ANDO KANAKO
(Sch. of Med., Kanazawa Univ.)
SHIRAO ETSUKO
(Sch. of Med., Kanazawa Univ.)
IWAKUCHI YUKARI
(Sch. of Med., Kanazawa Univ.)
SHIRAO YUTAKA
(Sch. of Med., Kanazawa Univ.)
INOUE AMANE
(Rohto Pharm. Co., Ltd.)
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Journal Title;Journal of Japanese Ophthalmological Society
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Journal Code:Z0666A
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ISSN:0029-0203
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VOL.104;NO.4;PAGE.207-213(2000)
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| Figure&Table&Reference;FIG.10, REF.14 |
| Pub. Country;Japan |
| Language;Japanese |
| Abstract;Purpose: We previously reported that a unique free fluorophore (Fl-Glc), presumably a .BETA.-glucoside, is particularly abundant in human brunescent cataractous lens nuclei. Our preliminary experiments indicated that incubation of low-molecular weight (MW) fraction of non-brunescent lens nuclei causes an increase in a particular fluorophore (Fl-X). This study was undertaken to compare the Fl-Glc with the Fl-X and subsequently to identify the Fl-X. Methods: Experiment 1) The purified Fl-X and its .BETA.-glucosidase digest (aglycon) were compared with the Fl-Glc and its aglycon, respectively, by high-performance liquid chromatography (HPLC). Experiment 2) i) The Fl-X and its aglycon were analysed by liquid chromatography/mass spectrometry (LC/MS). ii) Authentic xanthurenic acid was analysed by HPLC and LC/MS. Results: Experiment 1) The retention times of the Fl-X and the Fl-Glc exactly coincided. The fluorescence peaks of both disappeared after .BETA.-glucosidase treatment. Experiment 2) i) LC/MS results suggested that the MWs of the Fl-X and its aglycon were 367 and 205, respectively. ii) HPLC and LC/MS results for xanthurenic acid (MW=205) were exactly the same as those for the aglycon of the Fl-X. Conclusions: The Fl-Glc and the Fl-X are identical, and the Fl-X (=Fl-Glc) is a glucoside of xanthurenic acid. (author abst.) |
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