Classification of .BETA.-lactamases by utilizing the enzyme inhibitors.
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Accession number;00A0333859
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| Title;Classification of .BETA.-lactamases by utilizing the enzyme inhibitors. |
| Author;
SAWAI TETSUO
(Chiba Univ., Fac. of Pharm. Sci.)
OHARA KOJI
(Chiba Univ., Fac. of Pharm. Sci.)
ONUMA MASAE
(Chiba Univ., Fac. of Pharm. Sci.)
NAKAMURA AKIO
(Chiba Univ., Fac. of Pharm. Sci.)
NAITO YASUYO
(Chiba Univ., Fac. of Pharm. Sci.)
NAKAZAWA KYOKO
(Chiba Univ., Fac. of Pharm. Sci.)
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Journal Title;Japanese Journal of Chemotherapy
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Journal Code:F0608A
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ISSN:1340-7007
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VOL.48;NO.2;PAGE.101-106(2000)
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| Figure&Table&Reference;FIG.1, TBL.6, REF.19 |
| Pub. Country;Japan |
| Language;Japanese |
| Abstract;A simple and rapid method for classification of .BETA.-lactamases in bacterial cells into classes A, B, C and D was devised by utilizing three .BETA.-lactamase-inhibitors clavulanic acid, a carbapenem derivative (J-110,441) and a monobactam derivative (Syn-2161). On the basis of the combined effects of ampicillin with the inhibitor on the MIC of ampicillin, the enzyme class in the test organisms was estimated. This method was evaluated according to its application in 14 bacterial strains, which consisted of seven gram-negative species. For routine assay, a liquid microdilution method for MIC determination was employed. However, the time required for assay was reduced to one-fifth by employing an ATP-bioluminescens method developed by Hattori N, et al. (author abst.) |
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