A New Screening Method for Probiotic Lactic Acid Bacteria in the Lactobacillus acidophilus Group by Using Carbohydrate Probes.
|
Accession number;00A0211252
|
| Title;A New Screening Method for Probiotic Lactic Acid Bacteria in the Lactobacillus acidophilus Group by Using Carbohydrate Probes. |
| Author;
SAITO T
(Tohoku Univ., Sendai-shi, Jpn)
MATSUMURA A
(Tohoku Univ., Sendai-shi, Jpn)
KITAZAWA H
(Tohoku Univ., Sendai-shi, Jpn)
KAWAI Y
(Tohoku Univ., Sendai-shi, Jpn)
ITOH T
(Tohoku Univ., Sendai-shi, Jpn)
HASHIBA H
(Snow Brand Milk Products Co. Ltd., Kawagoe-shi, Jpn)
|
Journal Title;Anim Sci J
|
Journal Code:F0902A
|
ISSN:1344-3941
|
|
VOL.71;NO.1;PAGE.103-108(2000)
|
| Figure&Table&Reference;FIG.2, TBL.2, REF.10 |
| Pub. Country;Japan |
| Language;English |
| Abstract;To select probiotic strains of the Lactobacillus(L.) acidophilus group with high adhesion to human intestinal tracts for developing the functional yogurt, a new screening method using biotinylated carbohydrate probe(BCP) was introduced. After incubation of living cells of 30 strains of the L. acidophilus group with 5 kinds of BCPs which posses representative partial structures of the sugar chains constituting of human colonic mucin, the interaction between cell-surface lactins and BCP were evaluated. The highest reactivity was detected in 19 strains(about 63%) with a trisaccharide probe(GalNAc.ALPHA.(1.RAR.3)[Fuc.ALPHA.(1.RAR.2)]Gal.BETA.1.RAR.). The cell-surface lectin activity was first clearly confirmed in the B group of the L. acidophilus group. The adhesion evaluation score(AE score, total sum of each absorbance of 5 BCPs samples) is considered to be useful as an indicator for selection of the probiotic strains which have high adhesiveness in human gastrointestinal tract. (author abst.) |
|
|
|
Related Articles;
|
|
