The role of 17.BETA.-estradiol, progesterone, and human chorionic gonadotropin in in vitro decidualization of human endometrial stromal cells.
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Accession number;00A0358504
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| Title;The role of 17.BETA.-estradiol, progesterone, and human chorionic gonadotropin in in vitro decidualization of human endometrial stromal cells. |
| Author;
KASAHARA KYOKO
(Shiga Univ. of Med. Sci.)
TAKAKURA KENJI
(Shiga Univ. of Med. Sci.)
TAKEBAYASHI KOICHI
(Shiga Univ. of Med. Sci.)
KIMURA FUMINORI
(Shiga Univ. of Med. Sci.)
NAKANISHI KEIKO
(Shiga Univ. of Med. Sci.)
NODA YOICHI
(Shiga Univ. of Med. Sci.)
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Journal Title;Advances in Obstetrics and Gynecology
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Journal Code:Z0452B
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ISSN:0370-8446
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VOL.52;NO.2;PAGE.120-131(2000)
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| Figure&Table&Reference;FIG.8, REF.31 |
| Pub. Country;Japan |
| Language;Japanese |
| Abstract;The effects of gonadal steroids and human chorionic gonadotropin(hCG) on in vitro decidualization of human endometrial stromal cells(ESCs) were examined. Although widely known that human ESCs in culture express prolactin(PRL), a marker of decidualization, in response to gonadal steroids, there has been no report on the optimal 17.BETA.-estradiol (E2) and progesterone(P) concentrations for inducing in vitro decidualization. Also, while several authors have proposed that hCG can induce or promote in vitro decidualization of human ESCs, the concepts of these proposals differ from each other. Moreover, a recent study using the reverse-transcriptase mediated polymerase chain reaction(RT-PCR) has raised controversy over whether the hCG receptor is actually present or not in the human endometrium. Since hCG is not only a crucial hormone secreted by peri-implantation embryo but also often administered to infertile patients as luteal phase support, it is important to elucidate the effect of hCG on decidualization. Therefore, we investigated the optimal E2/P concentration for in vitro decidualization of human ESCs and the effects of hCG on human ESCs. We isolated and cultured human ESCs to which gonadal steroids were added, and we subsequently induced in vitro decidualization based on morphological change and increased PRL level in the media. Through a variety of experiments, the optimal E2/P concentration was determined to be 10nM and 100nM, respectively, and hCG neither induced in vitro decidualization of human ESCs by itself nor promoted in vitro decidualization induced by E2 and P. Moreover, a high dose (103-105mIU/ml) of hCG inhibited in vitro decidualization, without effecting cell proliferation. Dibutyryl-cAMP, a cAMP derivative, also failed to induce in vitro decidualization of ESCs, although some authors have reported that the endogenous production of cAMP is stimulated by hCG administration.... (author abst.) |
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