Gene Expression of NAD+-Dependent Sorbitol Dehydrogenase and NADP+-Dependent Sorbitol-6-Phosphate Dehydrogenase During Development of Loquat(Eriobotrya japonica Lindl.) Fruit.
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Accession number;00A0589123
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| Title;Gene Expression of NAD+-Dependent Sorbitol Dehydrogenase and NADP+-Dependent Sorbitol-6-Phosphate Dehydrogenase During Development of Loquat(Eriobotrya japonica Lindl.) Fruit. |
| Author;
BANTOG N A
(Nagoya Univ., Nagoya)
YAMADA K
(Nagoya Univ., Nagoya)
NIWA N
(Nagoya Univ., Nagoya)
SHIRATAKE K
(Nagoya Univ., Nagoya)
YAMAKI S
(Nagoya Univ., Nagoya)
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Journal Title;Journal of the Japanese Society for Horticultural Science
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Journal Code:F0626A
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ISSN:0013-7626
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VOL.69;NO.3;PAGE.231-236(2000)
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| Figure&Table&Reference;FIG.3, REF.26 |
| Pub. Country;Japan |
| Language;English |
| Abstract;The aim of this study is to clarify the roles of NAD+-dependent sorbitol dehydrogenase(NAD-SDH) and NAP+-dependent sorbitol-6-phosphate dehydrogenase(S6PDH) in fruit development, and the regulatory mechanism(s) underlying their expression during development using loquat as the study material. The cDNA of NAD-SDH, cloned from loquat fruit, consisted of 1,572bp and contained an open reading frame of 1,023bp capable of encoding a protein of 371 amino acids. The deduced amino acid sequence has 97.8% identity to that of an apple fruit. The activity of NAD-SDH is a function of fruit development, i.e. the increase in protein of this enzyme synchronized consistently with that of activity showing no posttranslational modification throughout the developmental stages. Furthermore, NAD-SDH activity correlated with the mRNA levels, indicating that the key regulatory step of the activity is at the transcriptional level. The increase in NAD-SDH with fruit development plays a dominantly important role during fruit maturation and sugar accumulation. The trend of S6PDH activity during fruit development paralleled that of NAD-SDH activity. It correlated with protein and mRNA levels revealing that the regulation of the activity is mainly at the transcriptional level like NAD-SDH. However, the role of S6PDH in fruit is not yet clear. (author abst.) |
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