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Accession number;00A0454436
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| Title;Characterization of Randomly Obtained cDNAs from Peach Fruits at Various Developmental Stages. |
| Author;
HAYAMA H
(National Inst. Fruit Tree Sci., Ibaraki)
SHIMADA T
(National Inst. Fruit Tree Sci., Ibaraki)
YAMAMOTO T
(National Inst. Fruit Tree Sci., Ibaraki)
IKETANI H
(National Inst. Fruit Tree Sci., Ibaraki)
MATSUTA N
(National Inst. Fruit Tree Sci., Ibaraki)
YOSHIOKA H
(National Inst. Fruit Tree Sci., Ibaraki)
HAYASHI T
(National Inst. Fruit Tree Sci., Ibaraki)
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Journal Title;Journal of the Japanese Society for Horticultural Science
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Journal Code:F0626A
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ISSN:0013-7626
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VOL.69;NO.2;PAGE.183-185(2000)
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| Figure&Table&Reference;TBL.2, REF.6 |
| Pub. Country;Japan |
| Language;English |
| Abstract;More than 200 cDNA clones were sequenced from cDNA libraries constructed from peach fruits sampled at three different developmental stages. For a rapid and simple construction of cDNA libraries, PCR amplification and plasmid vector were combined. Eighty-three clones (39% of the total number of clones) exhibited a significant homology (OPT scores.GEQ.200) to the registered sequences including Expressed Sequence Tags(ESTs). Almost all genes were newly obtained in peach (Prunus persica) as well as in other Prunus species. Random nucleotide sequencing of the cDNA libraries appeared to be a useful and valuable tool for understanding various physiological changes during fruit development in peach. (author abst.) |
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