Embryogenic Callus Induction and Plant Regeneration from Unfertilized Ovule of 'Kyoho' Grape.
|
Accession number;00A0454437
|
| Title;Embryogenic Callus Induction and Plant Regeneration from Unfertilized Ovule of 'Kyoho' Grape. |
| Author;
NAKAJIMA I
(National Inst. Fruit Tree Sci., Hiroshima)
KOBAYASHI S
(National Inst. Fruit Tree Sci., Hiroshima)
NAKAMURA Y
(National Inst. Fruit Tree Sci., Hiroshima)
|
Journal Title;Journal of the Japanese Society for Horticultural Science
|
Journal Code:F0626A
|
ISSN:0013-7626
|
|
VOL.69;NO.2;PAGE.186-188(2000)
|
| Figure&Table&Reference;FIG.1, TBL.1, REF.12 |
| Pub. Country;Japan |
| Language;English |
| Abstract;Unfertilized ovules were excised 19-21 days before anthesis and cultured in a liquid medium to induce embryogenic callus from 'Kyoho' grape (Vitis*labruscana). The culture medium consisted of half strength MS supplemented with 1.0.MU.M 2.4-dichlorophenoxyacetic acid (2,4-D) and 0.2-5.0.MU.M N-(1,2,3-thiadiazol-5-yl)-N'-phenylurea (TDZ, thidiazuron), or 1.0.MU.M 2,4-D and 0.2.MU.M N-(2-chloro-4-pyridyl)-N'-phenylurea (CPPU). After 3-4 months of culture, the embryogenic calli produced from the ovules were successfully subcultured to maintain a high embryogenic potential for over one year. When these adventitious embryos were transferred to a modified 1/2MS hormone-free medium, plantlets were regenerated 1-2.5 months later. This is the first embryogenic callus induction and plant regeneration from 'Kyoho' grape. (author abst.) |
|
|
|
Related Articles;
|
|
|
