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Accession number;00A0454438
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| Title;Visualization of Nucleus Substitution between Allium galanthum and Shallot (A. cepa) by Genomic In Situ Hybridization. |
| Author;
YAMASHITA K
(Saga Univ. Saga)
IINO M
(Saga Univ. Saga)
SHIGYO M
(Saga Univ. Saga)
TASHIRO Y
(Saga Univ. Saga)
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Journal Title;Journal of the Japanese Society for Horticultural Science
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Journal Code:F0626A
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ISSN:0013-7626
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VOL.69;NO.2;PAGE.189-191(2000)
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| Figure&Table&Reference;FIG.1, REF.9 |
| Pub. Country;Japan |
| Language;English |
| Abstract;Genomic in situ hybridization(GISH) was applied to trace the progress of nucleus substituion in continuous backcrossing for substituting the cytoplasm of Allium galanthum Kar. et Kir. for that of shallot(A. cepa L. Aggregatum group). Total genomic DNA of A. galanthum was used as a probe DNA along with that of shallot as a blocking DNA. In the F1 hybrid, eight chromosomes from A. galanthum were clearly discriminated from eight chromosomes from shallot. Chromosome regions from A. galanthum noticeably decreased in teh backcross progenies with frequency of backcrossing. This study demonstrates that GISH is a useful means of revealing the progress of nucleus substitution in the backcrossing. (author abst.) |
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