Isozymes and RAPD Markers to Identify the Parenthood of Japanese Pear 'Kuratsuki'.
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Accession number;00A0454442
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| Title;Isozymes and RAPD Markers to Identify the Parenthood of Japanese Pear 'Kuratsuki'. |
| Author;
BANNO KIYOSHI
(Shinshu Univ., Fac. of Agric.)
LIU Y
(Shinshu Univ., Fac. of Agric.)
ISHIKAWA HIRONOBU
(Shinshu Univ., Fac. of Agric.)
NAKANO SHIN'ICHI
(Ishikawaken Norinsuisambu)
NOBATAKE SHIGENORI
(Ishikawa Agriculture Res. Center, JPN)
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Journal Title;Journal of the Japanese Society for Horticultural Science
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Journal Code:F0626A
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ISSN:0013-7626
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VOL.69;NO.2;PAGE.208-213(2000)
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| Figure&Table&Reference;FIG.3, TBL.3, REF.12 |
| Pub. Country;Japan |
| Language;Japanese |
| Abstract;S-genotypes, isozyme phenotypes, and RAPD markers were investigated to identify the parenthood of the Japanese pear cultivar 'Kuratsuki', which is a chance seedling found in a mixed planting of 'Shinsui', 'Kosui', 'Hosui' and 'Chojuro'. The S-genotype of 'Kuratsuki' was judged to be S3S5, the same as that of 'Hosui', in that the pollen of 'Kuratsuki' was cross-incompatible, and it exhibited the same PCR-RFLP pattern of the S-RNase genes with 'Hosui'. Analyses of seven loci in five isozymes among the parental candidates showed that 'Kuratsuki' had the common a allele at POD-1 locus as 'Shinsui' and 'Chojuro', and the common b allele at PGM-3 locus comparable only to 'Hosui'. The common RAPD markers specific with 'Kuratsuki' were detected only in 'Shinsui' and 'Hosui' which suggest that 'Kuratsuki' is a hybrid seedling between 'Shinsui' and 'Hosui'. (author abst.) |
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