Mapping of neural cell and glia cell activity with PET.
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Accession number;00A0557804
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| Title;Mapping of neural cell and glia cell activity with PET. |
| Author;
INOUE OSAMU
(Osaka Univ., Med. Sch.)
KOBAYASHI KAORU
(Osaka Univ., Med. Sch.)
HOSOI RIE
(Osaka Univ., Med. Sch.)
ANDO KOICHI
(National Inst. of Radiological Sciences)
KOIKE SACHIKO
(National Inst. of Radiological Sciences)
TSUKADA HIDEO
(Hamamatsu Photonics K.K.)
GEE A
(Auhus Pet Centre)
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Journal Title;NIRS-M (Natl Inst Radiol Sci)
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Journal Code:Z0881A
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ISSN:
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VOL.;NO.133;PAGE.46-48(1999)
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| Figure&Table&Reference;FIG.4, TBL.2 |
| Pub. Country;Japan |
| Language;Japanese |
| Abstract;The left striatum of rat brain was lesioned with ibotenic acid, and in vivo autoradiographic study using 14C-benzylamine was performed 2 weeks after the lesion. Significant decreases in radioactivity concentration in the lesioned striatum were observed at both 1 min and 20 min after the tracer injection into MAO-inhibited rat. Together with the in vitro autoragiogram using brain slice indicated that binding sites for labeled amines in the brain might exist in neural cells rather than glial cell. The biodistribution studies using glioma bearing nude mice were also performed with 13N-ammonia, 14C-N, .ALPHA.-dimethyl benzylamine, 14C-deoxyglucose and 14C-methionine. Unexpectedly, relatively higher uptake of 14C-DMBA as compared with 13N-ammonia in glioma. For the estimation of the metabolic rate (k3) of 13N-ammonia in the brain with PET, the double tracer technique combined use of 15O-H2O and 13N-ammonia in the same rhesus monkey was applied. The input function of 13N-ammonia was determined by the rapid metabolite analysis of arterial plasma sample employing the evaporation method, which was established by HPLC analysis. Continuos infusion of 13N-ammonia was found to be better than the bolus injection in order to estimate k3 values. Two different compartment models were employed for the quantitative analysis of PET data. Three compartment model, which include the diffusion compartment, seemed to be better for the kinetic analysis of 13N-ammonia in the brain. (author abst.) |
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