Comparison of the Enzymatic Activities of Endo-1,4-.BETA.-Glucanases Derived from Clostridium josui.
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Accession number;00A0559774
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| Title;Comparison of the Enzymatic Activities of Endo-1,4-.BETA.-Glucanases Derived from Clostridium josui. |
| Author;
FUJINO E
(Mie Univ., Mie)
FUJINO T
(Nagoya Seiraku Co. Ltd., Aichi)
SAKKA K
(Mie Univ., Mie)
KIMURA K
(Mie Univ., Mie)
OHMIYA K
(Mie Univ., Mie)
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Journal Title;Bull Fac Bioresour Mie Univ
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Journal Code:Y0251B
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ISSN:0915-0471
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VOL.;NO.23;PAGE.23-30(1999)
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| Figure&Table&Reference;FIG.2, TBL.3, REF.21 |
| Pub. Country;Japan |
| Language;English |
| Abstract;Endoglucanase EG-1C, -2C and -3 (EC 3.2.1.4) derived from C. josui were highly active in degrading of carboxymethyl cellulose (CMC), but only slightly active on Avicel. All enzymes could not hydrolyze xylan and p-Nitrophenyl-.BETA.-D-glucoside. p-Nitrophenyl-.BETA.-D-cellobioside was degraded only by EG-1C. By CMC and cellooligosaccharide degrading analysis, the specific activity of EG-2C against CMC was 5 times higher than the others and its action mode was most endowisely. In degrading cello-oligosaccharides, these enzymes could degrade cellotetraose and cellopentaose, but not cellobiose and cellotriose. The specific activities increased as degree of polymerization increases from four to five. (author abst.) |
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