Genetic Analysis of Chicken Lines by Amplified Fragment Length Polymorphism (AFLP).
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Accession number;00A0535455
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| Title;Genetic Analysis of Chicken Lines by Amplified Fragment Length Polymorphism (AFLP). |
| Author;
LEE E J
(Kobe Univ., Kobe-shi, Jpn)
MANNEN H
(Kobe Univ., Kobe-shi, Jpn)
MIZUTANI M
(Nippon Inst. Biological Sci., Yamanashi-ken, Jpn)
TSUJI S
(Kobe Univ., Kobe-shi, Jpn)
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Journal Title;Anim Sci J
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Journal Code:F0902A
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ISSN:1344-3941
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VOL.71;NO.3;PAGE.231-238(2000)
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| Figure&Table&Reference;FIG.3, TBL.3, REF.14 |
| Pub. Country;Japan |
| Language;English |
| Abstract;The amplified fragment length polymorphism (AFLP) was applied to analyze genetic variation and relationships of eight chicken lines including Black Minorca (BM-C), White Leghorri (WL-GM) and Fayoumi (PNP, PNP/DO, PNN, GSN/1, GSN/2, and GSP) lines. Genomic DNA digested with EcoR I and Mse I was ligated to EcoR I and Mse I adapters and the fragments were amplified using four primer sets, each having three selective nucleotides at the 3' end. A fluorescent labeled Eco RI primer was used for the second amplification. A total of 188 distinctive bands were obtained with combinations of th f e our primer sets, of which 82 bands were polymorphic through the lines. The number of polymorphic bands between two lines were quite variable from 3 to 28. The average genetic variation expressed as band sharing (BS) values, ranged from 0.91 to 0.97 within chicken lines, while those values between breeds ranged from 0.37 to 0.51. A dendrogram constructed using the unweighted pair group method average (UPGMA) showed that these chicken lines could be classified into three breed-dependent groups and six Fayoumi lines were classified further into two sub-groups The fingerprinting of DNA by AFLP offers a powerful and convenient tool not only for assessment of inbreeding, but also for revealing genetic relationships between chicken lines prior to the construction of a resource family for linkage analysis. (author abst.) |
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