Science Links Japan | Complete Nucleotide Sequence of a Shiba Goat (Capra hircus) Heat-shock Protein 70-3 (Hsp 70-3) Gene.

Complete Nucleotide Sequence of a Shiba Goat (Capra hircus) Heat-shock Protein 70-3 (Hsp 70-3) Gene.

Accession number;00A0751184

Title;Complete Nucleotide Sequence of a Shiba Goat (Capra hircus) Heat-shock Protein 70-3 (Hsp 70-3) Gene.

Author; LUENGRATTANA Y (Tokyo Univ. Agriculture, Atsugi-shi, Jpn) KOHARA S (Tokyo Univ. Agriculture, Atsugi-shi, Jpn) HARA H (Tokyo Univ. Agriculture, Atsugi-shi, Jpn) MUKOYAMA H (Tokyo Univ. Agriculture, Atsugi-shi, Jpn) NANZAWA K (Tokyo Univ. Agriculture, Atsugi-shi, Jpn) WATANABE S (Tokyo Univ. Agriculture, Atsugi-shi, Jpn)

Journal Title;Anim Sci J

Journal Code:F0902A

ISSN:1344-3941

VOL.71;NO.4;PAGE.371-376(2000)

Figure&Table&Reference;FIG.3, TBL.2, REF.15

Pub. Country;Japan

Language;English

Abstract;The nucleotide sequence of a Shiba goat (Capra hircus) heat-shock protein 70-3 (Hsp 70 -3) gene was determined by the dideoxy-nucleotide chain termination method. The Hsp 70-3 gene was composed of an uninterrupted primary sequence of 2,696 nucleotides. It composed of a 5' noncoding leader sequence of 491 nucleotides, a 3' noncoding region of 294 nucleotides and a continuous open reading frame (ORF) of 1,911 nucleotides. The ORF encoded 636 amino acids and the predicted molecular mass was 69,863 daltons. The multiple potentially regulatory sequences i.e. TATA-box, CCAAT box, GC element and heat shock element (HSE), were found in the 5' flanking region of the goat Hsp 70-3 gene. Comparison of the predicted amino acid sequences of the goat Hsp 70-3 gene with the reported sequence a bovine Hsp 70-3 gene (GenBank accession No. L10428) revealed that the goat Hsp 70-3 was highly identical (up to 98.2%) to the bovine Hsp 70-3 protein, and only 6 out of 636 amino acids were different. The other minor difference was that the goat Hsp 70-3 protein had insertion of 5 amino acids (NKRAV) which were not observed in the bovine Hsp 70-3 protein. We concluded that Capra hircus Hsp 70-3 gene was the homologue of Bos taurus Hsp 70-3 gene considered as one member of the goat Hsp 70 gene family. (author abst.)

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