Development of Corneal Storage Medium. First Report. Examination of Rabbit Cornea.
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Accession number;01A0189817
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| Title;Development of Corneal Storage Medium. First Report. Examination of Rabbit Cornea. |
| Author;
TACHIBANA ATSUKO
(Nihon Univ., Sch. of Med.)
SAWA MITSURU
(Nihon Univ., Sch. of Med.)
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Journal Title;Journal of Japanese Ophthalmological Society
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Journal Code:Z0666A
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ISSN:0029-0203
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VOL.105;NO.1;PAGE.3-12(2001)
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| Figure&Table&Reference;FIG.2, TBL.1, REF.39 |
| Pub. Country;Japan |
| Language;Japanese |
| Abstract;Purpose: To develop and evaluate a new corneal storage medium with a simple formula by histological methods. Methods: We compared two corneal storage media, containing minimum essential medium (MEM) and 2.5% chondroitin sulfate, pH 7.33 and osmolality of 320 mOsm/kg, to OPTISOL-GS. The differences in the two media were the molecular weight (MW) and source of chondroitin sulfate. MW of Medium I was 27,500 and MW of Medium II was 33,700. A cornea with scleral rim obtained from a Japanese white rabbit was stored in either Medium I or Medium II and the fellow cornea was stored in OPTISOL-GSR for 7 or 14 days at 4.DEG.C.. Histological examination of corneal endothelial cells was performed both by scanning electron microscopy and by transmission electron microscopy. Results: At day 7, there was no significant difference in histological findings among the rabbit corneas stored in OPTISOL-GSR, Medium I, or Medium II. At. day 14, corneas stored in OPTISOL-GSR or Medium I showed similar histological findings. In Medium II, endothelial cells showed marked degen-eration. Conclusion: The results of experiments with rabbit cornea indicated that OPTISOL-GSR and Medium I could preserve endothelial cellular structure better than Medium II. The difference between Medium I and Medium II was only the MW of the chondroitin sulfate used. The MW may be an important factor to determine suitable chondroitin sulfate for developing a corneal storage medium. (author abst.) |
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