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Accession number;01A0228956
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| Title;Intramoleclar Fluorescence Resonance Energy Transfer System with Coumarin Donor Included in .BETA.-Cyclodextrin. |
| Author;
TAKAKUSA HIDEO
(Univ. of Tokyo, Grad. Sch.)
KIKUCHI KAZUYA
(Univ. of Tokyo, Grad. Sch.)
NAGANO TETSUO
(Univ. of Tokyo, Grad. Sch.)
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Journal Title;Abstracts. Symposium on Biofunctional Chemistry
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Journal Code:L0836A
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ISSN:
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VOL.15th;NO.;PAGE.28-29(2000)
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| Figure&Table&Reference;FIG.2 |
| Pub. Country;Japan |
| Language;Japanese |
| Abstract;The fluorescence resonance energy transfer (FRET) technique has been applied to probe biological systems and also for high-throughtput screening of combinatorial libraries, by means of fluorescence intensity ratio imaging. However, there are few examples of such FRET probes, because flexible linkers in aqueous solutions take conformations such that the donor and acceptor moieties are in close proximity, and the emissions of the fluorophores are quenched. In this study, we developed a conformationally flexible FRET system with coumarin donor included in .BETA.-CD usable in an aqueous environment without quenching of the two dyes. (author abst.) |
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