|
Accession number;01A0273753
|
| Title;Acute Ultraviolet B induced Lens Epithelial Cell Photo-damage and its Repair Process. |
| Author;
YAMADA YOSHIHISA
(Kanazawa Med. Univ.)
KOJIMA MASAMI
(Kanazawa Med. Univ.)
VRENSEN G F J M
(Netherlands Opthalmic Res. Inst.)
TAKAHASHI NOBUO
(Kanazawa Med. Univ.)
SASAKI KAZUYUKI
(Kanazawa Med. Univ.)
|
Journal Title;Journal of Japanese Ophthalmological Society
|
Journal Code:Z0666A
|
ISSN:0029-0203
|
|
VOL.105;NO.2;PAGE.102-110(2001)
|
| Figure&Table&Reference;FIG.5, REF.18 |
| Pub. Country;Japan |
| Language;Japanese |
| Abstract;Purpose: To clarify the process of acute lens epithelial cell photo-damage induced by ultraviolet B (UV-B) exposure and its repair. Methods: Pigmented rabbits were exposed to UV-B (300 mJ/cm2). The time course of lens epithelial cell photo-damage was evaluated by light microscopy of lens epithelial cell flat mounts. The flat mounted lens epithelial cells were stained with Mayer's hematoxylin or by immunohistochemistry with anti-proliferating cell nuclear antigen (PCNA) monoclonal antibodies. Results: The lens epithelial cells were irregularly arranged and there were debris and pycnotic nuclei, small nuclei, existence of large cells, and phagocytosis of neighboring cells in the pupillary area. A repair process was seen in the injured areas within a week after UV-B exposure. PCNA positive cells were seen and it was confirmed that the healing of the photo-injured part was due to epithelial cell proliferation. Conclusions: The acute lens epithelial cell photodamage induced by UV-B exposure was repaired within a week by cell proliferation. (author abst.) |
|
|
|
Related Articles;
|
|
